Evaluation of different preservation and storage methods for Malassezia spp.

نویسندگان

  • M J Crespo
  • M L Abarca
  • F J Cabañes
چکیده

Freezing at -80 degrees C, lyophilization, preservation in distilled water, and storage in different culture media were performed in order to find a suitable method that allowed a prolonged storage of Malassezia spp. Freezing at -80 degrees C was the only method successful at maintaining all species.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Glucose and lactose as cryoprotectants for fungal strains immobilised in sodium alginate: an emphasis on the conservation of the zygomycetes Rhizopus and Mucor.

This research aimed at investigating the cryoprotectant action of glucose and lactose on strains of Malassezia spp. and zygomycetes immobilised in sodium alginate. Twelve strains of Malassezia spp. (nine M. furfur, two M. globosa and one M. sympodialis) and 12 zygomycetes (five Rhizopus oryzae and seven Mucor hiemales) were immobilised in sodium alginate, within plastic beads, maintained in app...

متن کامل

Antifungal activity of herbal extracts against Malassezia species

Background: Malassezia spp. is an opportunistic dimorphicBasidiomycetous fungi associated with a variety of diseasesincluding dandruff, atopic eczema, pityriasis versicolor, seborrheicdermatitis and folliculitis. It also causes systemic infectionsin immune compromised patients. The aim of this study wasto evaluate the antimicrobial activity of nettle leaves (Urticadioica), colocynths fruits (Ci...

متن کامل

ارزیابی اثر داروهای ضد قارچی کتوکونازول و فلوکونازول روی گونه های مالاسزیا

Background and purpose: Malassezia, a yeast-like fungus found in normal skin flora is known to be associated with various skin diseases, along with systemic infections. Our aim was to determine the in -vitro susceptibility of Malassezia spp. to ketoconazole and fluconazole. Materials and methods: In this study, we identified 99 Malassezia isolates from patients with pityriasis vesicular by...

متن کامل

Rapid Identification of Malassezia furfur from other Malassezia Species: A Major Causative Agent of Pityriasis Versicolor

The present study is designed to evaluate the application of a simple method for rapid identification of Malassezia furfur among other Malassezia spp. based on production of a brownish-red pigment and its diffusion into the medium containing L-tryptophan as a sigle source of nitrogen.  91 strains of Malassezia (20% M. furfur, 2% M. sympodialis, 5% M. obtosa and 73% M. globosa) isolated from ski...

متن کامل

Association of Malassezia species with dandruff

BACKGROUND & OBJECTIVES Malassezia species implicated with dandruff vary at different geographical locations. The present study was conducted to determine the spectrum and distribution of Malassezia species in dandruff patients and healthy individuals. METHODS Patients with dandruff from northern (Chandigarh) and southern (Manipal, Karnataka) parts of India (50 each) and healthy individuals (...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Journal of clinical microbiology

دوره 38 10  شماره 

صفحات  -

تاریخ انتشار 2000